Product Information
KGF (FGF-7)
— Product Size —
KGF
10 μg (0.1 mg/ml, 100 μl)
SP-KGF-R-010UG
50 μg (0.1 mg/ml, 500 μl)
SP-KGF-R-050UG
1 mg (0.1 mg/ml, 10 ml)
SP-KGF-R-001MG
GMP compliant KGF
1 mg (0.1 mg/ml, 10 ml)
SP-KGF-G-001MG
Benefits
Significant cost reduction of cell
manufacturing processes
Seamless transition from basic research
to clinical applications
— Product Details —
| NAME | Recombinant Human KGF (FGF-7) |
|---|---|
| SOURCE | Corynebacterium glutamicum* |
| FORMULATION | Frozen in DPBS(-) containing 1.5mM DTT and 100mM Na2SO4 |
| Quality | Animal origin free |
| AA Sequence: | CNDMTPEQMA TNVNCSSPER HTRSYDYMEG GDIRVRRLFC RTQWYLRIDK RGKVKGTQEM KNNYNIMEIR TVAVGIVAIK GVESEFYLAM NKEGKLYAKK ECNEDCNFKE LILENHYNTY ASAKWTHNGG EMFVALNQKG IPVRGKKTKK EQKTAHFLPM AIT |
| Purity: | ≥ 95% by SDS-PAGE |
| Endotoxin | <0.1EU/ug |
| Description: | Keratinocyte Growth Factor (KGF) is one of 23 known members of the FGF family, and it is known as FGF-7. Proteins of this family play a central role during prenatal development, postnatal growth, and the regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. KGF is a mitogen factor specific for epithelial cells and keratinocytes. Recombinant Human KGF is an 18.9 kDa protein consisting of 163 amino acid residues. |
*Corynebacterium glutamicum
- • Discovered in 1957 as a glutamate producer
- • Fast growing soil bacterium
- • Gram-positive
- • Non-sporulating
- • Non-pathogenic
- • Non-endotoxic
- • FDA approved
< Features >
- Secretion System
- No Endotoxin
- Less Derivatives (impurities)
- Experience: Food Amino Acid Production
Ideal platform for low-cost, high-grade proteins production with high lot-to-lot consistency.
— Product Comparison Table —
| AJINOMOTO | Company A | Company B | |
|---|---|---|---|
| Host cell | C. glutamicum | E. coli | E. coli |
| Endotoxin | <0.1EU/μg (LAL) |
<1EU/μg (LAL) |
<0.10EU/μg (LAL) |
| Purity | ≧95% (SDS-PAGE) |
≧95% (SDS-PAGE、HPLC) |
>97% (SDS-PAGE) |
| Biological activity |
EC50, 0.1-30 ng/mL*1 | ED50, 0.1-15/ml*2 | ED50, 6-60 ng/ml*2 |
| Form | Frozen in DPBS(-) containing 1.5 mM DTT, 100 mM Na2SO4 |
Lyophilized | Lyophilized |
| N-terminal | (Cys)-Asn-Asp | − | Met |
| Expiration date | 3 years from manufacturing date |
− | 12 months from date of receipt |
As of 2022 Jan.
- *1 ERK1/2 phosphorylation assay using MCF-7 cells
- *2 Cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells
〈 Comparison of biological activity with other companies’ products 〉
Phosphorylation of ERK1/2
AJINOMOTO’s KGF demonstrates equal biological
activity compared to tested alternatives!
〈 Stability information after reconstitution 〉
| AJINOMOTO | Company A | Company B | |
|---|---|---|---|
| -80°C | 3 years from manufacturing date |
3 months from reconstitution date |
3 months from reconstitution date (-20 to -70 °C) |
| 4°C | ー | 1 week from reconstitution date |
1 month from reconstitution date |
| Freeze-thaw cycles |
Within 3 times* | ー | ー |
As of 2022 Jan.
- * We do not guarantee the product quality.
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・Product Information
KGF ［1mg GMP complinat］(PDF [131KB]) KGF ［1mg］(PDF [277KB]) KGF ［10μg］(PDF [282KB]) KGF ［50μg］(PDF [280KB])・SDS (PDF [128KB] / En) (PDF [143KB] / JP)
For more information, please contact us.
15-1, Kyobashi 1-Chome, Chuo-Ku, Tokyo 104-8315, Japan
