Product Information
SCF
— Product Size —
10 μg (0.1 mg/ml, 100 μl)
SP-SCF-R-010UG
50 μg (0.1 mg/ml, 500 μl)
SP-SCF-R-050UG
1 mg (0.1 mg/ml, 10 ml)
SP-SCF-R-001MG
GMP compliant product is in preparation. Contact us for further information.
Benefits
Significant cost reduction of cell
manufacturing processes
Seamless transition from basic research
to clinical applications
— Product Details —
| NAME | Recombinant Human SCF |
|---|---|
| SOURCE | Corynebacterium glutamicum* |
| FORMULATION | Frozen in 20 mM HEPES |
| Quality | Animal origin free |
| AA Sequence: | EGICRNRVTN NVKDVTKLVA NLPKDYMITL KYVPGMDVLP SHCWISEMVV QLSDSLTDLL DKFSNISEGL SNYSIIDKLV NIVDDLVECV KENSSKDLKK SFKSPEPRLF TPEEFFRIFN RSIDAFKDFV VASETSDCVV SSTLSPEKDS RVSVTKPFML PPVA |
| Purity: | ≥ 95% by SDS-PAGE |
| Endotoxin | <0.1EU/ug |
| Description: | SCF (stem cell factor) is a growth factor secreted from fibroblasts and endothelial cells. SCF binds to a receptor known as c-Kit and is thought to play an important role in the survival, proliferation and differentiation of hematopoietic stem cells. Recombinant Human SCF is an 18.5 kDa polypeptide containing 164 amino acid residues, which corresponds to the sequence of the secreted soluble form of SCF. |
*Corynebacterium glutamicum
- • Discovered in 1957 as a glutamate producer
- • Fast growing soil bacterium
- • Gram-positive
- • Non-sporulating
- • Non-pathogenic
- • Non-endotoxic
- • FDA approved
< Features >
- Secretion System
- No Endotoxin
- Less Derivatives (impurities)
- Experience: Food Amino Acid Production
Ideal platform for low-cost, high-grade proteins production with high lot-to-lot consistency.
— Product Comparison Table —
| AJINOMOTO | Company A | Company B | Company C | |
|---|---|---|---|---|
| Host cell | C. glutamicum | E. coli | E. coli | E. coli |
| Endotoxin | <0.1EU/μg (LAL) |
<1EU/μg (LAL) |
<1.0EU/μg (LAL) |
<1EU/μg (LAL) |
| Purity | ≧95% (SDS-PAGE) |
≧98% (SDS-PAGE、HPLC) |
>97% (SDS-PAGE) |
>97% (SDS-PAGE) |
| Biological activity |
EC50, 1.0-20 ng/mL*1 | ED50, ≦2.0 ng/ml*2 | ED50, ≦1-5 ng/ml*2 | ED50, ≦5 ng/ml*2 |
| Form | Frozen (in 20mM HEPES) |
Lyophilized | Lyophilized | Lyophilized |
| N-terminal | Glu-Gly-Ile | ー | Met | ー |
| Expiration date | 3 years from manufacturing date |
ー | 12 months from date of receipt |
ー |
As of 2021 Oct.
- *1 Phosphorylation assay of c-KIT
- *2 Cell proliferation assay using TF‑1 human erythroleukemic cells
〈 Comparison of biological activity with other companies’ products 〉
Phosphorylation assay of c-KIT
AJINOMOTO’s SCF demonstrates equal to biological
activity compared to tested alternatives!
〈 Stability information after reconstitution 〉
| AJINOMOTO | Company A | Company B | |
|---|---|---|---|
| -80°C | 3 years from manufacturing date |
1 year from reconstitution date |
3 months from reconstitution date |
| 4°C | At least 1 month* | 1 month from reconstitution date |
1 month from reconstitution date |
| Freeze-thaw cycles | Within 3 times* | ー | ー |
As of 2021 Oct.
- * We do not guarantee the product quality.
- Download
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・Product Information
SCF ［1mg］(PDF [261KB]) SCF ［10μg］(PDF [266KB]) SCF ［50μg］(PDF [266KB])・SDS (PDF [127KB] / En) (PDF [138KB] / JP)
For more information, please contact us.
15-1, Kyobashi 1-Chome, Chuo-Ku, Tokyo 104-8315, Japan
