Key points for single-cell cloning with coating-free method

Key points for successful single-cell passage

Key point for making cell stocks

Development for StemFit media

• Sci.Rep. 2014 Jan 8;4:3594.
  A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells.

Maintenance protocol for primate PSCs with StemFit medium

• Sci Data. 2017 Jun 20;4:170067.
  Single-cell Transcriptome of Early Embryos and Cultured Embryonic Stem Cells of Cynomolgus Monkeys
• Sci Rep. 2018 Aug 15;8(1):12187
  Derivation of Induced Pluripotent Stem Cells in Japanese Macaque (Macaca Fuscata)

Application for genome-edited clone generation

• Methods. 2016 May 15;101:43-55.
  Engineering the AAVS1 locus for consistent and scalable transgene expression in human iPSCs and their differentiated derivatives.

Liver & Gut organoids

• Cell Rep. 2017 Dec 5;21(10):2661-2670.
  Massive and Reproducible Production of Liver Buds Entirely from Human Pluripotent Stem Cells.
• Nature. 2017 Jun 22;546(7659):533-538.
  Multilineage communication regulates human liver bud development from pluripotency.
• Stem Cell Reports. 2018 Mar 13;10(3):780-793.
  Human iPSC-Derived Posterior Gut Progenitors Are Expandable and Capable of Forming Gut and Liver Organoids.
• Nature, 499, 481-484 (2013)
  Takebe, T. et al., Vascularized and functional human liver from an iPSC-derived organ bud transplant.
• Cell Rep., 21, 2661-2670 (2017)
  Takebe, T. et al., Massive and Reproducible Production of Liver Buds Entirely https://www.cell.com/stem-cell-reports/pdf/S2213-6711(18)30278-9.pdf　from Human Pluripotent Stem Cells.
• STEM CELL REPORTS, 11, 306-316 (2018)
  Takebe, H. et al., Optimal Hypoxia Regulates Human iPSC-Derived Liver Bud Differentiation through Intercellular TGFβ Signaling.
• Stabilized generation of human iPSC-derived liver organoids using a modified coating approach
• Periodic formation of epithelial somites from human pluripotent stem cell

Kidney organoids

• Cell Stem Cell. 2017 Dec 7;21(6):730-746.e6.
  Higher-Order Kidney Organogenesis from Pluripotent Stem Cells.
• Methods Mol Biol. 2017;1597:179-193.
  Generation of a Three-Dimensional Kidney Structure from Pluripotent Stem Cells.

iPSC establishment

• An induced pluripotent stem cell line (EHTJUi003-A) generated from a neonate with c.1377delC mutation in the gene MYBPC3 causing hypertrophic cardiomyopathy
• Xeno-free Reprogramming of Peripheral Blood Mononuclear Erythroblasts on Laminin 521.

Differentiation

• PLoS One. 2014 Dec 2;9(12):e112291.
  Derivation of mesenchymal stromal cells from pluripotent stem cells through a neural crest lineage using small molecule compounds with defined media.
• Stem Cell Res. 2017 Dec;25:98-106.
  A human iPS cell myogenic differentiation system permitting high-throughput drug screening.
• Stem Cell Reports. 2017 Nov 14;9(5):1406-1414.
  Efficient Large-Scale 2D Culture System for Human Induced Pluripotent Stem Cells and Differentiated Cardiomyocytes.
• Stem Cell Reports. 2016 Mar 8;6(3):312-20.
  Cardiomyocytes Derived from MHC-Homozygous Induced Pluripotent Stem Cells Exhibit Reduced Allogeneic Immunogenicity in MHC-Matched Non-human Primates.
• Nature. 2017 Aug 30;548(7669):592-596.
  Human iPS cell-derived dopaminergic neurons function in a primate Parkinson's disease model.
• Mol Brain. 2016 Sep 19;9(1):85.
  Pathological classification of human iPSC-derived neural stem/progenitor cells towards safety assessment of transplantation therapy for CNS diseases.

Mesenchymal Stem Cell Differentiatoin

• Selective vulnerability of human-induced pluripotent stem cells to dihydroorotate dehydrogenase inhibition during mesenchymal stem/stromal cell purification
• In vivo regeneration of rat laryngeal cartilage with mesenchymal stem cells derived from human induced pluripotent stem cells via neural crest cells
• Pro‑angiogenic scafold‑free Bio three‑dimensional conduit developed from human induced pluripotent stem cell‑derived mesenchymal stem cells promotes peripheral nerve regeneration